Objective : To evaluate the change in heart rate during the reversal of neuromuscular blockade by using 3 dosages of atropine in a mixture with neostigmine. Methods : Sixty patients with ASA physical status I or II undergoing balanced general anesthesia were randomly allocated into 3 groups. Neostigmine 0.05 mg/kg mixed with atropine 0.01, 0.015, and 0.02 mg/kg were given intravenously in group A, B, and C respectively at the time of reversal of neuromuscular blockade.Results : In the first 5 minutes, all groups manifested with an initial increase in heart rate (P = 0.008, P = 0.007 and P = 0.008 respectively) however, group B and C showed more tachycardia than group A (P = 0.028 and P = 0.021 respectively) After 15 minutes, heart rate became stable and returned close to the baseline. Conclusion : In healthy patients, atropine 0.01 mg/kg is safe for the reversal of neuromuscular blockade since it affects the increase in heart rate very little as compare to the conventional dose of atropine.